Gastric lavage as a non-lethal method for stingray (Myliobatiformes) diet sampling

Abstract

Understanding the trophic interactions of ecologically important mesopredators such as Myliobatiformes (stingrays) is vital, but, given their susceptibility to overexploitation, the collection of stomach contents for dietary analysis necessitates the use of non-lethal methods. We provide a detailed method of using gastric lavage to collect stomach contents from the ‘Vulnerable’ porcupine ray Urogymnus asperrimus at the St Joseph Atoll of the Amirantes Group, Seychelles. Rays were captured by hand, inverted and restrained while a plastic hose, connected to a bilge pump, was inserted into the stomach to flush out the contents. A high percentage (94.5%) of gastric lavage attempts resulted in the collection of stomach contents. There was no visual evidence of stress or short-term, post-release mortality and, given the success and relative ease of this technique, we conclude that the described method of gastric lavage is an effective, non-lethal means of obtaining stomach contents from stingrays.